StepOnePlus real-time PCR

Instrument:

StepOnePlus real-time PCR

Contact:

Birgitte Hønsvall

Technology Description:

"Bertha" is our StepOnePlus machine for real-time/quantitative PCR. 

The StepOnePlus can do both real-time runs or end-point runs.

Bertha is connected to a laptop with the StepOne software. It is also possible to run Bertha as a stand-alonge system.

 

The system has four filters that can detect these fluorophores:

Filter Dye emission
1

FAM

SYBR Green

~ 520 nm
2

JOE

VIC

~ 550 nm
3

TAMRA

NED

~ 580 nm
4 ROX ~ 610 nm

 

Extensive manuals for the following types of experiments are found in the biology lab's index file:

  • Standard curve experiments
  • Relative standard curve and comparative CT experiments
  • Genotyping experiments
  • Precence/absence experiments

Technical Information:

Category: Chemical and Biological methods Area name: Biology Model: StepOnePlus Manufacturer: Applied Biosystems