Instrument: |
Fluorescence microscope |
Contact: |
Amin Hossein Zavieh
Ken Roger Ervik |
Technology Description: |
The Zeiss Axio Scope.A1 is an optical microscope that can be used with both reflected and transmitted light. It is a highly flexible setup specifically intended for biological samples. The microscope can be used with large samples (up to 110mm wide and 380mm high!). The setup has filters for: * Bright field (BF): illumination from below * Epi bright field (Epi BF): illumination from above * Dark field (DF): only observing light scattered by the specimen * Fluorescence with up to three dyes: - Fs 49: excitation at 365nm (105nm bandpass width) - Fs 38: excitation at 470nm (40nm bandpass width) - Fs 20: excitation at 546nm (12nm bandpass width) * Differential interference contrast (DIC): relief contrast for unstained samples
The microscope has three interchangeable light sources: * 2x Halogen lamps for both transmitted and reflected light * A HXP 120W mercury arc lamp for fluorescence excitation
The objective lenses installed are: * 10x and 20x N-ACHROPLAN inexpensive all-round objectives with good chromatic correction and planarity. * 5x and 20x EC Plan-NEOFLUAR and 40x LD Plan-NEOFLUAR high-resolution objectives for high contrast and high transmission. * 63x Acroplan oil immersion objective
Images can be captured on the connected computer using the AxioCam MRc firewire camera (1388x1040 pixel sensor) mounted on top of the microscope. |
Technical Information: |
Category: Characterization Area name: Bionano Model: Axio Scope.A1 430035-9120-000 Manufacturer: Carl Zeiss |
